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Antimicrobial activities of a promising glycolipid biosurfactant from a novel marine Staphylococcus saprophyticus SBPS 15.

ABSTRACT: Biosurfactants have gained a renewed interest in the recent years for their commercial application in diverse research areas. Recent evidences suggest that the antimicrobial activities exhibited by biosurfactants make them promising molecules for the application in the field of therapeutics. Marine microbes are well known for their unique metabolic and functional properties; however, few reports are available till date regarding their biosurfactant production and antimicrobial potential. In an ongoing survey for bioactive microbial metabolites from microbes isolated from diverse ecological niches, a marine Staphylococcus saprophyticus SBPS 15 isolated from the petroleum hydrocarbon contaminated coastal site, Puducherry, India, was identified as a promising biosurfactant producer based on multiple screening methods. This bacterium exhibited growth-dependent biosurfactant production and the recorded yield was 1.345 ± 0.056 g/L (on dry weight basis). The biosurfactant was purified and chemically characterized as a glycolipid with a molecular mass of 606.7 Da, based on TLC, biochemical estimation methods, FT-IR spectrum and MALDI-TOF-MS analysis. Further, the estimated molecular mass was different from the earlier reports on biosurfactants. This new glycolipid biosurfactant exhibited a board range of pH and temperature stability. Furthermore, it revealed a promising antimicrobial activity against many tested human pathogenic bacterial and fungal clinical isolates. Based on these observations, the isolated biosurfactant from the marine S. saprophyticus revealed board physicochemical stabilities and possess excellent antimicrobial activities which proves its significance for possible use in various therapeutic and biomedical applications. To the best of our knowledge, this is the first report of a biosurfactant from the bacterium, S. saprophyticus.

SUBMITTER: Mani P

PROVIDER: S-EPMC4978644 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Antimicrobial activities of a promising glycolipid biosurfactant from a novel marine Staphylococcus saprophyticus SBPS 15.

Mani P P Dineshkumar G G Jayaseelan T T Deepalakshmi K K Ganesh Kumar C C Senthil Balan S S

3 Biotech 20160809 2

Biosurfactants have gained a renewed interest in the recent years for their commercial application in diverse research areas. Recent evidences suggest that the antimicrobial activities exhibited by biosurfactants make them promising molecules for the application in the field of therapeutics. Marine microbes are well known for their unique metabolic and functional properties; however, few reports are available till date regarding their biosurfactant production and antimicrobial potential. In an o ...[more]

PMID: 28330235

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Project description:Biosurfactants derived from different microbes are an alternative to chemical surfactants, which have broad applications in food, oil, biodegradation, cosmetic, agriculture, pesticide and medicine/pharmaceutical industries. This is due to their environmentally friendly, biocompatible, biodegradable, effectiveness to work under various environmental conditions and non-toxic nature. Lactic acid bacteria (LAB)-derived glycolipid biosurfactants can play a major role in preventing bacterial attachment, biofilm eradication and related infections in various clinical settings and industries. Hence, it is important to explore and identify the novel molecule/method for the treatment of biofilms of pathogenic bacteria. In the present study, a probiotic Lactobacillus rhamnosus(L. rhamnosus) strain was isolated from human breast milk. Firstly, its ability to produce biosurfactants, and its physicochemical and functional properties (critical micelle concentration (CMC), reduction in surface tension, emulsification index (% EI24), etc.) were evaluated. Secondly, inhibition of bacterial adhesion and biofilm eradication by cell-bound biosurfactants from L. rhamnosus was performed against various biofilm-forming pathogens (B. subtilis, P. aeruginosa, S. aureus and E. coli). Finally, bacterial cell damage, viability of cells within the biofilm, exopolysaccharide (EPS) production and identification of the structural analogues of the crude biosurfactant via gas chromatography-mass spectrometry (GC-MS) analysis were also evaluated. As a result, L. rhamnosus was found to produce 4.32 ± 0.19 g/L biosurfactant that displayed a CMC of 3.0 g/L and reduced the surface tension from 71.12 ± 0.73 mN/m to 41.76 ± 0.60 mN/m. L. rhamnosus cell-bound crude biosurfactant was found to be effective against all the tested bacterial pathogens. It displayed potent anti-adhesion and antibiofilm ability by inhibiting the bacterial attachment to surfaces, leading to the disruption of biofilm formation by altering the integrity and viability of bacterial cells within biofilms. Our results also confirm the ability of the L. rhamnosus cell-bound-derived biosurfactant to damage the architecture of the biofilm matrix, as a result of the reduced total EPS content. Our findings may be further explored as a green alternative/approach to chemically synthesized toxic antibiofilm agents for controlling bacterial adhesion and biofilm eradication.

Dataset Information

Antimicrobial activities of a promising glycolipid biosurfactant from a novel marine Staphylococcus saprophyticus SBPS 15.

Publications

Antimicrobial activities of a promising glycolipid biosurfactant from a novel marine Staphylococcus saprophyticus SBPS 15.

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