Ontology highlight
ABSTRACT:
SUBMITTER: Stirman JN
PROVIDER: S-EPMC4980167 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Stirman Jeffrey N JN Smith Ikuko T IT Kudenov Michael W MW Smith Spencer L SL
Nature biotechnology 20160627 8
Two-photon calcium imaging provides an optical readout of neuronal activity in populations of neurons with subcellular resolution. However, conventional two-photon imaging systems are limited in their field of view to ∼1 mm(2), precluding the visualization of multiple cortical areas simultaneously. Here, we demonstrate a two-photon microscope with an expanded field of view (>9.5 mm(2)) for rapidly reconfigurable simultaneous scanning of widely separated populations of neurons. We custom designed ...[more]