Ontology highlight
ABSTRACT:
SUBMITTER: Chen HJ
PROVIDER: S-EPMC4980997 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Nature biotechnology 20160711 8
Refined cancer models are needed to bridge the gaps between cell line, animal and clinical research. Here we describe the engineering of an organotypic colon cancer model by recellularization of a native human matrix that contains cell-populated mucosa and an intact muscularis mucosa layer. This ex vivo system recapitulates the pathophysiological progression from APC-mutant neoplasia to submucosal invasive tumor. We used it to perform a Sleeping Beauty transposon mutagenesis screen to identify g ...[more]