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Visualizing fungal metabolites during mycoparasitic interaction by MALDI mass spectrometry imaging.

ABSTRACT: Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized confrontation assay. By this we were able to visualize metabolite distributions by MALDI MSI after matrix deposition with a home-built sublimation device and thorough recrystallization. We present for the first time MALDI MSI data for secondary metabolite release during active mycoparasitism.

SUBMITTER: Holzlechner M

PROVIDER: S-EPMC4982067 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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Visualizing fungal metabolites during mycoparasitic interaction by MALDI mass spectrometry imaging.

Holzlechner Matthias M Reitschmidt Sonja S Gruber Sabine S Zeilinger Susanne S Marchetti-Deschmann Martina M

Proteomics 20160413 11-12

Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized confrontation assay. By this we were able to visualize metabolite distributions by MALDI MSI after matrix deposition with a home-built sublimation device and thorough recrystallizat ...[more]

PMID: 26959280

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