Genetic characterization of fluoroquinolone-resistant Escherichia coli associated with bovine mastitis in India.

ABSTRACT:

Aim

The present study was undertaken to characterize the mutation in gyrA (DNA gyrase) and parC (topoisomerase IV) genes responsible for fluoroquinolone resistance in Escherichia coli isolates associated with the bovine mastitis.

Materials and methods

A total of 92 milk samples from bovine mastitis cases were sampled in and around Puducherry (Southern India). Among these samples, 30 isolates were bacteriologically characterized as E. coli. Minimum inhibitory concentrations (MIC) of fluoroquinolones of these 30 E. coli isolates were evaluated by resazurin microtiter assay. Then, the quinolone resistance determining region (QRDR) (gyrA and parC genes) of these E. coli isolates was genetically analyzed for determining the chromosomal mutation causing fluoroquinolone resistance.

SUBMITTER: Balakrishnan S 

PROVIDER: S-EPMC4983120 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature