Unknown

Dataset Information

0

Forkhead box O member FOXO1 regulates the majority of follicle-stimulating hormone responsive genes in ovarian granulosa cells.

ABSTRACT: FSH promotes maturation of ovarian follicles. One pathway activated by FSH in granulosa cells (GCs) is phosphatidylinositol-3 kinase/AKT. The AKT target FOXO1 is reported to function primarily as a repressor of FSH genes, including Ccnd2 and Inha. Based on its broad functions in other tissues, we hypothesized that FOXO1 may regulate many more GC genes. We transduced GCs with empty adenovirus or constitutively active FOXO1 followed by treatment with FSH for 24 h, and conducted RNA deep sequencing. Results show that FSH regulates 3772 genes ?2.0-fold; 60% of these genes are activated or repressed by FOXO1. Pathway Studio Analysis revealed enrichment of genes repressed by FOXO1 in metabolism, signaling, transport, development, and activated by FOXO1 in signaling, cytoskeletal functions, and apoptosis. Gene regulation was verified by q-PCR (eight genes) and ChIP analysis (two genes). We conclude that FOXO1 regulates the majority of FSH target genes in GCs.

SUBMITTER: Herndon MK 

PROVIDER: S-EPMC4983523 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Forkhead box O member FOXO1 regulates the majority of follicle-stimulating hormone responsive genes in ovarian granulosa cells.

Herndon Maria K MK   Law Nathan C NC   Donaubauer Elyse M EM   Kyriss Brandon B   Hunzicker-Dunn Mary M  

Molecular and cellular endocrinology 20160617

FSH promotes maturation of ovarian follicles. One pathway activated by FSH in granulosa cells (GCs) is phosphatidylinositol-3 kinase/AKT. The AKT target FOXO1 is reported to function primarily as a repressor of FSH genes, including Ccnd2 and Inha. Based on its broad functions in other tissues, we hypothesized that FOXO1 may regulate many more GC genes. We transduced GCs with empty adenovirus or constitutively active FOXO1 followed by treatment with FSH for 24 h, and conducted RNA deep sequencing  ...[more]

Similar Datasets

Unknown Involvement of FoxO1 in the effects of follicle-stimulating hormone on inhibition of apoptosis in mouse granulosa cells.
| S-EPMC4237239 | biostudies-literature
Unknown Follicle-stimulating hormone and luteinizing hormone increase Ca2+ in the granulosa cells of mouse ovarian follicles†.
| S-EPMC7302518 | biostudies-literature
Unknown Follicle-Stimulating Hormone Glycobiology.
| S-EPMC6534497 | biostudies-literature
Unknown Administration of follicle-stimulating hormone induces autophagy via upregulation of HIF-1? in mouse granulosa cells.
| S-EPMC5596559 | biostudies-literature
Unknown Metformin inhibits follicle-stimulating hormone (FSH) action in human granulosa cells: relevance to polycystic ovary syndrome.
| S-EPMC3784648 | biostudies-literature
Unknown Canonical WNT signaling inhibits follicle stimulating hormone mediated steroidogenesis in primary cultures of rat granulosa cells.
| S-EPMC3895028 | biostudies-literature
Unknown Pharmacogenetics of follicle-stimulating hormone action.
| S-EPMC3348539 | biostudies-literature
Unknown Profile of follicle-stimulating hormone and polymorphism of follicle-stimulating hormone receptor in Madrasin cattle with ovarian hypofunction.
| S-EPMC7311873 | biostudies-literature
Unknown Constitutively active protein kinase A qualitatively mimics the effects of follicle-stimulating hormone on granulosa cell differentiation.
| S-EPMC2725770 | biostudies-other
Unknown A DNA topoisomerase I inhibitor blocks the differentiation of rat granulosa cells induced by follicle-stimulating hormone.
| S-EPMC1151270 | biostudies-other