Flexible analysis of digital PCR experiments using generalized linear mixed models.
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ABSTRACT:
SUBMITTER: Vynck M
PROVIDER: S-EPMC4983648 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Publications
Flexible analysis of digital PCR experiments using generalized linear mixed models.
Vynck Matthijs M Vandesompele Jo J Nijs Nele N Menten Björn B De Ganck Ariane A Thas Olivier O
Biomolecular detection and quantification 20160624
The use of digital PCR for quantification of nucleic acids is rapidly growing. A major drawback remains the lack of flexible data analysis tools. Published analysis approaches are either tailored to specific problem settings or fail to take into account sources of variability. We propose the generalized linear mixed models framework as a flexible tool for analyzing a wide range of experiments. We also introduce a method for estimating reference gene stability to improve accuracy and precision of ...[more]
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