Efficient Genome Editing in Apple Using a CRISPR/Cas9 system.
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ABSTRACT:
SUBMITTER: Nishitani C
PROVIDER: S-EPMC4987624 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Publications
Efficient Genome Editing in Apple Using a CRISPR/Cas9 system.
Nishitani Chikako C Hirai Narumi N Komori Sadao S Wada Masato M Okada Kazuma K Osakabe Keishi K Yamamoto Toshiya T Osakabe Yuriko Y
Scientific reports 20160817
Genome editing is a powerful technique for genome modification in molecular research and crop breeding, and has the great advantage of imparting novel desired traits to genetic resources. However, the genome editing of fruit tree plantlets remains to be established. In this study, we describe induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the CRISPR/Cas9 system. Four guide RNAs (gRNAs) were designed and stably transformed with Cas9 separately i ...[more]
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