Ontology highlight
ABSTRACT:
SUBMITTER: Sasano Y
PROVIDER: S-EPMC4987674 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Sasano Yu Y Nagasawa Koki K Kaboli Saeed S Sugiyama Minetaka M Harashima Satoshi S
Scientific reports 20160817
PCR-mediated chromosome splitting (PCS) was developed in the yeast Saccharomyces cerevisiae. It is based on homologous recombination and enables division of a chromosome at any point to form two derived and functional chromosomes. However, because of low homologous recombination activity, PCS is limited to a single site at a time, which makes the splitting of multiple loci laborious and time-consuming. Here we have developed a highly efficient and versatile chromosome engineering technology name ...[more]