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Cre-dependent DREADD (Designer Receptors Exclusively Activated by Designer Drugs) mice.

ABSTRACT: DREADDs, designer receptors exclusively activated by designer drugs, are engineered G protein-coupled receptors (GPCR) which can precisely control GPCR signaling pathways (for example, Gq, Gs, and Gi). This chemogenetic technology for control of GPCR signaling has been successfully applied in a variety of in vivo studies, including in mice, to remotely control GPCR signaling, for example, in neurons, glia cells, pancreatic ?-cells, or cancer cells. In order to fully explore the in vivo applications of the DREADD technology, we generated hM3Dq and hM4Di strains of mice which allow for Cre recombinase-mediated restricted expression of these pathway-selective DREADDs. With the many Cre driver lines now available, these DREADD lines will be applicable to studying a wide array of research and preclinical questions. genesis 54:439-446, 2016. © 2016 Wiley Periodicals, Inc.

SUBMITTER: Zhu H

PROVIDER: S-EPMC4990490 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature

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Cre-dependent DREADD (Designer Receptors Exclusively Activated by Designer Drugs) mice.

Zhu Hu H Aryal Dipendra K DK Olsen Reid H J RH Urban Daniel J DJ Swearingen Amanda A Forbes Stacy S Roth Bryan L BL Hochgeschwender Ute U

Genesis (New York, N.Y. : 2000) 20160603 8

DREADDs, designer receptors exclusively activated by designer drugs, are engineered G protein-coupled receptors (GPCR) which can precisely control GPCR signaling pathways (for example, Gq, Gs, and Gi). This chemogenetic technology for control of GPCR signaling has been successfully applied in a variety of in vivo studies, including in mice, to remotely control GPCR signaling, for example, in neurons, glia cells, pancreatic β-cells, or cancer cells. In order to fully explore the in vivo applicati ...[more]

PMID: 27194399

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Designer Receptors Exclusively Activated by Designer Drugs Approach to Treatment of Sleep-disordered Breathing.

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