Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Hydrodynamic dispensing and electrical manipulation of attolitre droplets.

ABSTRACT: Dispensing and manipulation of small droplets is important in bioassays, chemical analysis and patterning of functional inks. So far, dispensing of small droplets has been achieved by squeezing the liquid out of a small orifice similar in size to the droplets. Here we report that instead of squeezing the liquid out, small droplets can also be dispensed advantageously from large orifices by draining the liquid out of a drop suspended from a nozzle. The droplet volume is adjustable from attolitre to microlitre. More importantly, the method can handle suspensions and liquids with viscosities as high as thousands mPa?s markedly increasing the range of applicable liquids for controlled dispensing. Furthermore, the movement of the dispensed droplets is controllable by the direction and the strength of an electric field potentially allowing the use of the droplet for extracting analytes from small sample volume or placing a droplet onto a pre-patterned surface.

SUBMITTER: Zhang Y

PROVIDER: S-EPMC4990644 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Hydrodynamic dispensing and electrical manipulation of attolitre droplets.

Zhang Yanzhen Y Zhu Benliang B Liu Yonghong Y Wittstock Gunther G

Nature communications 20160812

Dispensing and manipulation of small droplets is important in bioassays, chemical analysis and patterning of functional inks. So far, dispensing of small droplets has been achieved by squeezing the liquid out of a small orifice similar in size to the droplets. Here we report that instead of squeezing the liquid out, small droplets can also be dispensed advantageously from large orifices by draining the liquid out of a drop suspended from a nozzle. The droplet volume is adjustable from attolitre ...[more]

PMID: 27514279

Similar Datasets

Acoustic Manipulation of Droplets under Reduced Gravity.

Project description:Contactless manipulation of matter is essential for studying physical phenomena. Acoustic manipulation of liquid samples using ultrasonic phased arrays provides a novel and attractive solution for mid-air manipulation, such as levitation, transportation, coalescence, mixing, separation, evaporation, and extraction, with a simple and single sequence. Despite the importance of gravity in droplet dynamics, its effect on a levitated droplet with an ultrasonic phased array remains unclear. To disseminate acoustic manipulation, better understanding of the fundamental physics of a droplet manipulated by ultrasonic phased arrays is required. Here, we show contactless levitation, transportation, and coalescence of multiple droplets under both ground and reduced gravity. Under ground gravity, the possible levitation size of the sample is limited to below the half wavelength of sound. Under reduced gravity, however, droplets that are larger than the limit can be successfully levitated, transported, and coalesced. Furthermore, the threshold of sound pressure for droplet levitation and manipulation could be minimised with the suppression of nonlinear acoustic phenomena under reduced gravity. These insights promote the development of contactless manipulation techniques of droplets for future space experiment and inhabitancy.

| S-EPMC6851086 | biostudies-literature

Manipulation of coacervate droplets with an electric field.

Project description:Many biopolymers are highly charged, and as in the case of many polymer mixtures, they tend to phase separate as a natural consequence of chain connectivity and an associated relatively low entropy of polymer mixing. Recently, it has become appreciated that the phase-separated structures formed by such polyelectrolyte blends, called "complex coacervates," underlie numerous biological structures and processes essential to living systems, and there has been intense interest in understanding the unique physical features of this type of phase-separation process. In the present work, we are particularly concerned with the field responsiveness of stabilized coacervate droplets formed after the phase separation of polyelectrolyte blend solution and then exposed to deionized water, making the droplet interfacial layer acquire a viscoelastic character that strongly stabilizes it against coalescence. We show that we can precisely control the positions of individual droplets and arrays of them with relatively low-voltage electric fields (on the order of 10 V/cm) and that the imposition of an oscillatory field gives rise to chain formation with coarsening of these chains into long fibers. Such a phase-separation-like process is generally observed in electrorheological fluids of solid colloidal particles subjected to much larger field strengths. The key to these coacervates' electrorheological properties is the altered interfacial viscoelastic properties when the droplets are introduced into deionized water and the associated high polarizability of the droplets, similar to the properties of many living cells. Since many different molecular payloads can be incorporated into these stable droplets, we anticipate many applications.

| S-EPMC9372540 | biostudies-literature

Electroactive hydrodynamic weirs for microparticle manipulation and patterning.

Project description:We present a platform for parallelized manipulations of individual polarizable micron-scale particles (i.e., microparticles) that combines negative dielectrophoretic forcing with the passive capture of hydrodynamic weir-based trapping. Our work enables manipulations using ejection- andor exclusion-based methods. In ejection operations, we unload targeted weirs by displacing microparticles from their capture faces via electrode activation. In exclusion-based operations, we prevent weir loading by activating selected on-chip electrodes before introducing microparticles into the system. Our work describes the device's passive loading dynamics and demonstrates enhanced functionalities by forming a variety of particle patterns.

| S-EPMC2682758 | biostudies-other

Precise pooling and dispensing of microfluidic droplets towards micro- to macro-world interfacing.

Project description:Droplet microfluidics possesses unique properties such as the ability to carry out multiple independent reactions without dispersion of samples in microchannels. We seek to extend the use of droplet microfluidics to a new range of applications by enabling its integration into workflows based on traditional technologies, such as microtiter plates. Our strategy consists in developing a novel method to manipulate, pool and deliver a precise number of microfluidic droplets. To this aim, we present a basic module that combines droplet trapping with an on-chip valve. We quantitatively analyzed the trapping efficiency of the basic module in order to optimize its design. We also demonstrate the integration of the basic module into a multiplex device that can deliver 8 droplets at every cycle. This device will have a great impact in low throughput droplet applications that necessitate interfacing with macroscale technologies. The micro- to macro- interface is particularly critical in microfluidic applications that aim at sample preparation and has not been rigorously addressed in this context.

| S-EPMC4251704 | biostudies-literature

Spontaneous electrical charging of droplets by conventional pipetting.

Project description:We report that a droplet dispensed from a micropipette almost always has a considerable electrical charge of a magnitude dependent on the constituents of the droplet, on atmospheric humidity and on the coating material of pipette tip. We show that this natural electrification of a droplet originates from the charge separation between a droplet and pipette tip surface by contact with water due to the ionization of surface chemical groups. Charge on a droplet can make it difficult to detach the droplet from the pipette tip, can decrease its surface tension, can affect the chemical characteristics of solutions due to interactions with charged molecules, and can influence the combination and localization of charged bio-molecules; in all cases, the charge may affect results of experiments in which any of these factors is important. Thus, these findings reveal experimental parameters that should be controlled in experiments that use micropipettes.

| S-EPMC3687225 | biostudies-literature

An Integrated Microfluidic SELEX Approach Using Combined Electrokinetic and Hydrodynamic Manipulation.

Project description:This article presents a microfluidic approach for the integration of the process of aptamer selection via systematic evolution of ligands by exponential enrichment (SELEX). The approach employs bead-based biochemical reactions in which affinity-selected target-binding oligonucleotides are electrokinetically transferred for amplification, while the amplification product is transferred back for affinity selection via pressure-driven fluid flow. The hybrid approach simplifies the device design and operation procedures by reduced pressure-driven flow control requirements and avoids the potentially deleterious exposure of targets to electric fields prior to and during affinity selection. In addition, bead-based reactions are used to achieve the on-chip coupling of affinity selection and amplification of target-binding oligonucleotides, thereby realizing on-chip loop closure and integration of the entire SELEX process without requiring offline procedures. The microfluidic approach is thus capable of closed-loop, multiround aptamer enrichment as demonstrated by selection of DNA aptamers against the protein immunoglobulin E with high affinity ( KD = 12 nM) in a rapid manner (4 rounds in approximately 10 h).

| S-EPMC5417355 | biostudies-literature

Hydrodynamic manipulation of nano-objects by optically induced thermo-osmotic flows.

Project description:Manipulation of nano-objects at the microscale is of great technological importance for constructing new functional materials, manipulating tiny amounts of fluids, reconfiguring sensor systems, or detecting tiny concentrations of analytes in medical screening. Here, we show that hydrodynamic boundary flows enable the trapping and manipulation of nano-objects near surfaces. We trigger thermo-osmotic flows by modulating the van der Waals and double layer interactions at a gold-liquid interface with optically generated local temperature fields. The hydrodynamic flows, attractive van der Waals and repulsive double layer forces acting on the suspended nanoparticles enable precise nanoparticle positioning and guidance. A rapid multiplexing of flow fields permits the parallel manipulation of many nano-objects and the generation of complex flow fields. Our findings have direct implications for the field of plasmonic nanotweezers and other thermo-plasmonic trapping systems, paving the way for nanoscopic manipulation with boundary flows.

| S-EPMC8813924 | biostudies-literature

Dynamic manipulation of droplets using mechanically tunable microtextured chemical gradients.

Project description:Materials and strategies applicable to the dynamic transport of microdroplets are relevant to surface fluidics, self-cleaning materials, thermal management systems, and analytical devices. Techniques based on electrowetting, topographic micropatterns, and thermal/chemical gradients have advanced considerably, but dynamic microdroplet transport remains a challenge. This manuscript reports the fabrication of mechano-tunable, microtextured chemical gradients on elastomer films and their use in controlled microdroplet transport. Specifically, discreet mechanical deformations of these films enabled dynamic tuning of the microtextures and thus transport along surface-chemical gradients. The interplay between the driving force of the chemical gradient and the microtopography was characterized, facilitating accurate prediction of the conditions (droplet radius and roughness) which supported transport. In this work, the use of microtextured surface chemical gradients in mechano-adaptive materials with microdroplet manipulation functionality was highlighted.

| S-EPMC8149645 | biostudies-literature

Remote Manipulation of Droplets on a Flexible Magnetically Responsive Film.

Project description:The manipulation of droplets is used in a wide range of applications, from lab-on-a-chip devices to bioinspired functional surfaces. Although a variety of droplet manipulation techniques have been proposed, active, fast and reversible manipulation of pure discrete droplets remains elusive due to the technical limitations of previous techniques. Here, we describe a novel technique that enables active, fast, precise and reversible control over the position and motion of a pure discrete droplet with only a permanent magnet by utilizing a magnetically responsive flexible film possessing actuating hierarchical pillars on the surface. This magnetically responsive surface shows reliable actuating capabilities with immediate field responses and maximum tilting angles of ~90°. Furthermore, the magnetic responsive film exhibits superhydrophobicity regardless of tilting angles of the actuating pillars. Using this magnetically responsive film, we demonstrate active and reversible manipulation of droplets with a remote magnetic force.

| S-EPMC4673453 | biostudies-literature

Indirect optical trapping using light driven micro-rotors for reconfigurable hydrodynamic manipulation.

Project description:Optical tweezers are a highly versatile tool for exploration of the mesoscopic world, permitting non-contact manipulation of nanoscale objects. However, direct illumination with intense lasers restricts their use with live biological specimens, and limits the types of materials that can be trapped. Here we demonstrate an indirect optical trapping platform which circumvents these limitations by using hydrodynamic forces to exert nanoscale-precision control over aqueous particles, without directly illuminating them. Our concept is based on optically actuated micro-robotics: closed-loop control enables highly localised flow-fields to be sculpted by precisely piloting the motion of optically-trapped micro-rotors. We demonstrate 2D trapping of absorbing particles which cannot be directly optically trapped, stabilise the position and orientation of yeast cells, and demonstrate independent control over multiple objects simultaneously. Our work expands the capabilities of optical tweezers platforms, and represents a new paradigm for manipulation of aqueous mesoscopic systems.

| S-EPMC6418258 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data