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Yeast life-span extension by calorie restriction is independent of NAD fluctuation.

ABSTRACT: Calorie restriction (CR) slows aging in numerous species. In the yeast Saccharomyces cerevisiae, this effect requires Sir2, a conserved NAD+-dependent deacetylase. We report that CR reduces nuclear NAD+ levels in vivo. Moreover, the activity of Sir2 and its human homologue SIRT1 are not affected by physiological alterations in the NAD+:NADH ratio. These data implicate alternate mechanisms of Sir2 regulation by CR.

SUBMITTER: Anderson RM

PROVIDER: S-EPMC4998187 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature

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Yeast life-span extension by calorie restriction is independent of NAD fluctuation.

Anderson Rozalyn M RM Latorre-Esteves Magda M Neves Ana Rute AR Lavu Siva S Medvedik Oliver O Taylor Christopher C Howitz Konrad T KT Santos Helena H Sinclair David A DA

Science (New York, N.Y.) 20031106 5653

Calorie restriction (CR) slows aging in numerous species. In the yeast Saccharomyces cerevisiae, this effect requires Sir2, a conserved NAD+-dependent deacetylase. We report that CR reduces nuclear NAD+ levels in vivo. Moreover, the activity of Sir2 and its human homologue SIRT1 are not affected by physiological alterations in the NAD+:NADH ratio. These data implicate alternate mechanisms of Sir2 regulation by CR. ...[more]

PMID: 14605207

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Sir2-independent life span extension by calorie restriction in yeast.

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Calorie restriction-mediated replicative lifespan extension in yeast is non-cell autonomous.

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| S-EPMC4310591 | biostudies-other

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