Ontology highlight
ABSTRACT:
SUBMITTER: El-Gaby M
PROVIDER: S-EPMC4999416 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
El-Gaby Mohamady M Zhang Yu Y Wolf Konstantin K Schwiening Christof J CJ Paulsen Ole O Shipton Olivia A OA
Cell reports 20160811 8
Tools that allow acute and selective silencing of synaptic transmission in vivo would be invaluable for understanding the synaptic basis of specific behaviors. Here, we show that presynaptic expression of the proton pump archaerhodopsin enables robust, selective, and reversible optogenetic synaptic silencing with rapid onset and offset. Two-photon fluorescence imaging revealed that this effect is accompanied by a transient increase in pH restricted to archaerhodopsin-expressing boutons. Cruciall ...[more]