Ontology highlight
ABSTRACT:
SUBMITTER: Lee SY
PROVIDER: S-EPMC4999972 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Lee Song-Yi SY Lee Hakbong H Lee Hye-Kyeong HK Lee Seung-Won SW Ha Sung Chul SC Kwon Taejoon T Seo Jeong Kon JK Lee Changwook C Rhee Hyun-Woo HW
ACS central science 20160812 8
Mammalian target of rapamycin (mTOR) signaling is a core pathway in cellular metabolism, and control of the mTOR pathway by rapamycin shows potential for the treatment of metabolic diseases. In this study, we employed a new proximity biotin-labeling method using promiscuous biotin ligase (pBirA) to identify unknown elements in the rapamycin-induced interactome on the FK506-rapamycin binding (FRB) domain in living cells. FKBP25 showed the strongest biotin labeling by FRB-pBirA in the presence of ...[more]