Dataset Information

Isolation and Characterization of a Glycosyl Hydrolase Family 16 ?-Agarase from a Mangrove Soil Metagenomic Library.

ABSTRACT: A mangrove soil metagenomic library was constructed and a ?-agarase gene designated as AgaML was isolated by functional screening. The gene encoded for a 659-amino-acids polypeptide with an estimated molecular mass of 71.6 kDa. The deduced polypeptide sequences of AgaML showed the highest identity of 73% with the glycoside hydrolase family 16 ?-agarase from Microbulbifer agarilyticus in the GenBank database. AgaML was cloned and highly expressed in Escherichia coli BL21(DE3). The purified recombinant protein, AgaML, showed optimal activity at 50 °C and pH 7.0. The kinetic parameters of Km and Vmax values toward agarose were 4.6 mg·mL(-1) and 967.5 ?M·min(-1)·mg(-1), respectively. AgaML hydrolyzed the ?-1,4-glycosidic linkages of agar to generate neoagarotetraose (NA4) and neoagarohexaose (NA6) as the main products. These characteristics suggest that AgaML has potential application in cosmetic, pharmaceuticals and food industries.

SUBMITTER: Mai Z

PROVIDER: S-EPMC5000755 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature

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Isolation and Characterization of a Glycosyl Hydrolase Family 16 β-Agarase from a Mangrove Soil Metagenomic Library.

Mai Zhimao Z Su Hongfei H Zhang Si S

International journal of molecular sciences 20160819 8

A mangrove soil metagenomic library was constructed and a β-agarase gene designated as AgaML was isolated by functional screening. The gene encoded for a 659-amino-acids polypeptide with an estimated molecular mass of 71.6 kDa. The deduced polypeptide sequences of AgaML showed the highest identity of 73% with the glycoside hydrolase family 16 β-agarase from Microbulbifer agarilyticus in the GenBank database. AgaML was cloned and highly expressed in Escherichia coli BL21(DE3). The purified recomb ...[more]

PMID: 27548158

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Dataset Information

Isolation and Characterization of a Glycosyl Hydrolase Family 16 ?-Agarase from a Mangrove Soil Metagenomic Library.

Publications

Isolation and Characterization of a Glycosyl Hydrolase Family 16 β-Agarase from a Mangrove Soil Metagenomic Library.

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