Direct detection of nitrotyrosine-containing proteins using an aniline-based oxidative coupling strategy.
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ABSTRACT:
SUBMITTER: Sangsuwan R
PROVIDER: S-EPMC5001876 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Publications
Direct detection of nitrotyrosine-containing proteins using an aniline-based oxidative coupling strategy.
Sangsuwan Rapeepat R Obermeyer Allie C AC Tachachartvanich Phum P Palaniappan Krishnan K KK Francis Matthew B MB
Chemical communications (Cambridge, England) 20160722 65
A convenient two-step method is described for the detection of nitrotyrosine-containing proteins. First, nitrotyrosines are reduced to aminophenols using sodium dithionite. Following this, an oxidative coupling reaction is used to attach anilines bearing fluorescence reporters or affinity probes. Features of this approach include fast reaction times, pmol-level sensitivity, and excellent chemoselectivity. ...[more]
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