Ontology highlight
ABSTRACT:
SUBMITTER: Castello A
PROVIDER: S-EPMC5003815 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Castello Alfredo A Fischer Bernd B Frese Christian K CK Horos Rastislav R Alleaume Anne-Marie AM Foehr Sophia S Curk Tomaz T Krijgsveld Jeroen J Hentze Matthias W MW
Molecular cell 20160721 4
Mammalian cells harbor more than a thousand RNA-binding proteins (RBPs), with half of these employing unknown modes of RNA binding. We developed RBDmap to determine the RNA-binding sites of native RBPs on a proteome-wide scale. We identified 1,174 binding sites within 529 HeLa cell RBPs, discovering numerous RNA-binding domains (RBDs). Catalytic centers or protein-protein interaction domains are in close relationship with RNA-binding sites, invoking possible effector roles of RNA in the control ...[more]