Topoisomerase II beta interacts with cohesin and CTCF at topological domain borders.

ABSTRACT:

Background

Type II DNA topoisomerases (TOP2) regulate DNA topology by generating transient double stranded breaks during replication and transcription. Topoisomerase II beta (TOP2B) facilitates rapid gene expression and functions at the later stages of development and differentiation. To gain new insight into the genome biology of TOP2B, we used proteomics (BioID), chromatin immunoprecipitation, and high-throughput chromosome conformation capture (Hi-C) to identify novel proximal TOP2B protein interactions and characterize the genomic landscape of TOP2B binding at base pair resolution.

Results

Our human TOP2B proximal protein interaction network included members of the cohesin complex and nucleolar proteins associated with rDNA biology. TOP2B associates with DNase I hypersen

SUBMITTER: Uuskula-Reimand L 

PROVIDER: S-EPMC5006368 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature