A spectrum of CodY activities drives metabolic reorganization and virulence gene expression in Staphylococcus aureus.

ABSTRACT: The global regulator CodY controls the expression of dozens of metabolism and virulence genes in the opportunistic pathogen Staphylococcus aureus in response to the availability of isoleucine, leucine and valine (ILV), and GTP. Using RNA-Seq transcriptional profiling and partial activity variants, we reveal that S. aureus CodY activity grades metabolic and virulence gene expression as a function of ILV availability, mediating metabolic reorganization and controlling virulence factor production in vitro. Strains lacking CodY regulatory activity produce a PIA-dependent biofilm, but development is restricted under conditions that confer partial CodY activity. CodY regulates the expression of thermonuclease (nuc) via the Sae two-component system, revealing cascading virulence regulation and fa

SUBMITTER: Waters NR 

PROVIDER: S-EPMC5007081 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature