Association between ABCB1 C3435T polymorphism and breast cancer risk: a Moroccan case-control study and meta-analysis.

ABSTRACT: Breast cancer is the most common cause of cancer death among women. Several studies have investigated the relationship between the C3435T polymorphism of ABCB1 gene and risk of breast cancer; but the results are conflicting. In the present study, we sought to assess the relationship between the C3435T polymorphism in ABCB1 gene and the risk of breast cancer in a sample of the Moroccan population.A case control study was performed on 60 breast cancer patients and 68 healthy women. The ABCB1 C3435T polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Furthermore, a meta-analysis including 16 studies with 6094 cases of breast cancer and 8646 controls was performed.Genotype frequencies were 50 % for CC, 33.3 % for CT and 16.7 % for TT in patients and 41.2 % for CC, 48.5 % for CT and 10.3 % for TT respectively in the control group. This difference was not statistically significant. The same trend as observed in the allele distribution between patients and controls (P?=?0.84). Findings from the meta-analysis showed that the ABCB1 C3435T polymorphism was not associated with an increased risk of breast cancer in the dominant model (OR?=?0.907; 95 % CI?=?0.767-1.073; P?=?0.25) as well as in the recessive model (OR?=?1.181; 95 % CI?=?0.973-1.434; P?=?0.093) and in the allele contrast model (OR?=?1.098; 95 % CI?=?0.972-1.240; P?=?0.133). However, the stratification of studies on ethnic basis showed that the TT genotype was associated with the risk of breast cancer in Asians (OR?=?1.405; 95 % CI?=?1.145-1.725; P?=?0.001), Caucasians (OR?=?1.093; 95 % CI?=?1.001-1.194; P?=?0.048) and North African (OR?=?2.028; 95 % CI?=?1.220-3.371; P?=?0.006).We have noted that the implication of C3435T variant on the risk of breast cancer was ethnicity-dependent. However, there is no evidence that ABCB1 C3435T polymorphism could play a role in susceptibility to breast cancer in Morocco. Further studies with a larger sample size, extended to other polymorphisms are needed to understand the influence of ABCB1 genetic variants on the risk of breast cancer.

SUBMITTER: Tazzite A 

PROVIDER: S-EPMC5007843 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature