Dataset Information

Gene-expression analysis of clozapine treatment in whole blood of patients with psychosis.

ABSTRACT:

Objectives

Clozapine is an atypical antipsychotic primarily prescribed for treatment-resistant schizophrenia. We tested the specific effect of clozapine versus other drug treatments on whole-blood gene expression in a sample of patients with psychosis from the UK.

Methods

A total of 186 baseline whole-blood samples from individuals receiving treatment for established psychosis were analysed for gene expression on Illumina HumanHT-12.v4 BeadChips. After standard quality-control procedures, 152 samples remained, including 55 from individuals receiving clozapine. In a within-case study design, weighted gene correlation network analysis was used to identify modules of coexpressed genes. The influence of mood stabilizers, lithium carbonate/lithium citrate and sodium valproate was studied to identify their possible roles as confounders.

Results

Individuals receiving clozapine as their only antipsychotic (clozapine monotherapy) had a nominal association with one gene-expression module, whereas no significant change in gene expression was found for other drugs.

Conclusion

Overall, this study does not provide evidence that clozapine treatment induces medium to large different gene-expression patterns in human whole blood versus other antipsychotic treatments. This does not rule out the possibility of smaller effects as observed for other common antipsychotic treatments.

SUBMITTER: Harrison RN

PROVIDER: S-EPMC5010277 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Gene-expression analysis of clozapine treatment in whole blood of patients with psychosis.

Harrison Rebecca N S RN Murray Robin M RM Lee Sang Hyuck SH Paya Cano Jose J Dempster David D Curtis Charles J CJ Dima Danai D Gaughran Fiona F Breen Gerome G de Jong Simone S

Psychiatric genetics 20161001 5

<h4>Objectives</h4>Clozapine is an atypical antipsychotic primarily prescribed for treatment-resistant schizophrenia. We tested the specific effect of clozapine versus other drug treatments on whole-blood gene expression in a sample of patients with psychosis from the UK.<h4>Methods</h4>A total of 186 baseline whole-blood samples from individuals receiving treatment for established psychosis were analysed for gene expression on Illumina HumanHT-12.v4 BeadChips. After standard quality-control pro ...[more]

PMID: 27315048

Similar Datasets

Project description:RNA samples from the blood of patients with double tumors, single tumors and from healthy control subjects are studied by gene expression analysis using the whole genome microarray platform AB1700 from Applied Biosystems. Gene expression profiles are derived from the microarray raw data by biostatistical analysis. Differential gene expression between the study subgroups and categories is assessed: On the one hand, all possible pairwise comparisons between the subgroups A, B, C, D, E and N are made in order to elucidate characteristics of the individual subgroups. On the other hand, the higher-ranked categories “double cancer” (AB, consisting of subgroups A and B) and “single cancer” (category CDE, consisting of subgroups C, D and E) are compared to each other and to the normal controls (N). Finally, the category “double cancer” is compared to the different single cancer subgroups (C, D and E). Analysis on the level of the higher ranked categories “double cancer” and “single cancer” aims at identifying the common traits of patients who have developed two independent tumors, as opposed to patients with only one tumor entity and to healthy individuals. Based on the functional annotation of the induced and repressed genes found in these comparisons, biological processes and pathways which are differentially activated in the subgroups or categories are identified, thus providing insights into their molecular characteristics. Furthermore, gene expression data are used to derive classifier rules, which are aimed at discriminating subjects from the different subgroups and categories and may constitute the basis for a future diagnostic classification tool. Keywords: microarray analysis, double-single primary malignancies A total of 66 individuals were analyzed:The primary study group consisted of 24 patients with two primary malignancies (group AB) (one of the primaries being of the breast, colon or ovary). The four control groups consisted of a total of 33 single cancer patiens: 10 with colon cancer (group C), 14 with breast cancer (group D), 9 with ovarian cancer (group E_) and 10 healthy individuals (group N). 4 samples (GECM-061_14, GECM-061_37, GECM-061_54 and GECM-061_72) were identifed as outliers by MA plots and therfore they were excluded from the study.

Dataset Information

Gene-expression analysis of clozapine treatment in whole blood of patients with psychosis.

Objectives

Methods

Results

Conclusion

Publications

Gene-expression analysis of clozapine treatment in whole blood of patients with psychosis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets