Dataset Information

Biodegradation of isoproturon by Pseudoxanthomonas sp. isolated from herbicide-treated wheat fields of Tarai agro-ecosystem, Pantnagar.

ABSTRACT: A gram-negative, rod-shaped, isoproturon (IPU) utilizing bacterium was isolated from herbicide-applied wheat fields of Tarai agro-ecosystem, Pantnagar. The phylogenetic sequence analysis based on 16S rRNA sequence revealed that the isolate could be a distinct species within the genus Pseudomonas. The isolate was a close relative of Pseudoxanthomonas japonensis (95 % similarity) and designated as K2. The bacterial isolate showed positive reaction for oxidase, catalase, and 20 carbohydrates using KB009 Part A and B HiCarbohydrate™ Kit. Degradation experiments were conducted using 200 mg l-1 initial IPU as a source of carbon at different pH and temperatures. Maximum IPU degradation by K2 was observed at pH 7.0 and 30 °C, while least degradation at 6.5 pH and 25 °C. Addition of dextrose along with IPU as an auxiliary carbon source increased IPU degradation by 4.72 %, as compared to the IPU degradation without dextrose under optimum conditions. 4-isopropylaniline was detected as a degradation by-product in the medium. The present study demonstrated the IPU metabolizing capacity of a novel bacterial isolate K2 that can be a better choice for the remediation of IPU-contaminated sites.

SUBMITTER: Giri K

PROVIDER: S-EPMC5010538 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Biodegradation of isoproturon by Pseudoxanthomonas sp. isolated from herbicide-treated wheat fields of Tarai agro-ecosystem, Pantnagar.

Giri Krishna K Pandey Shailseh S Kumar Rajesh R Rai J P N JP

3 Biotech 20160902 2

A gram-negative, rod-shaped, isoproturon (IPU) utilizing bacterium was isolated from herbicide-applied wheat fields of Tarai agro-ecosystem, Pantnagar. The phylogenetic sequence analysis based on 16S rRNA sequence revealed that the isolate could be a distinct species within the genus Pseudomonas. The isolate was a close relative of Pseudoxanthomonas japonensis (95 % similarity) and designated as K2. The bacterial isolate showed positive reaction for oxidase, catalase, and 20 carbohydrates using ...[more]

PMID: 28330262

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Project description:Soil organic carbon (SOC) is an important and manageable property of soils that impacts on multiple ecosystem services through its effect on soil processes such as nitrogen (N) cycling and soil physical properties. There is considerable interest in increasing SOC concentration in agro-ecosystems worldwide. In some agro-ecosystems, increased SOC has been found to enhance the provision of ecosystem services such as the provision of food. However, increased SOC may increase the environmental footprint of some agro-ecosystems, for example by increasing nitrous oxide emissions. Given this uncertainty, progress is needed in quantifying the impact of increased SOC concentration on agro-ecosystems. Increased SOC concentration affects both N cycling and soil physical properties (i.e., water holding capacity). Thus, the aim of this study was to quantify the contribution, both positive and negative, of increased SOC concentration on ecosystem services provided by wheat agro-ecosystems. We used the Agricultural Production Systems sIMulator (APSIM) to represent the effect of increased SOC concentration on N cycling and soil physical properties, and used model outputs as proxies for multiple ecosystem services from wheat production agro-ecosystems at seven locations around the world. Under increased SOC, we found that N cycling had a larger effect on a range of ecosystem services (food provision, filtering of N, and nitrous oxide regulation) than soil physical properties. We predicted that food provision in these agro-ecosystems could be significantly increased by increased SOC concentration when N supply is limiting. Conversely, we predicted no significant benefit to food production from increasing SOC when soil N supply (from fertiliser and soil N stocks) is not limiting. The effect of increasing SOC on N cycling also led to significantly higher nitrous oxide emissions, although the relative increase was small. We also found that N losses via deep drainage were minimally affected by increased SOC in the dryland agro-ecosystems studied, but increased in the irrigated agro-ecosystem. Therefore, we show that under increased SOC concentration, N cycling contributes both positively and negatively to ecosystem services depending on supply, while the effects on soil physical properties are negligible.

Dataset Information

Biodegradation of isoproturon by Pseudoxanthomonas sp. isolated from herbicide-treated wheat fields of Tarai agro-ecosystem, Pantnagar.

Publications

Biodegradation of isoproturon by Pseudoxanthomonas sp. isolated from herbicide-treated wheat fields of Tarai agro-ecosystem, Pantnagar.

Similar Datasets

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