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Activation of ?-adrenergic receptors is required for elevated ?1A-adrenoreceptors expression and signaling in mesenchymal stromal cells.


ABSTRACT: Sympathetic neurons are important components of mesenchymal stem cells (MSCs) niche and noradrenaline regulates biological activities of these cells. Here we examined the mechanisms of regulation of MSCs responsiveness to noradrenaline. Using flow cytometry, we demonstrated that ?1A adrenergic receptors isoform was the most abundant in adipose tissue-derived MSCs. Using calcium imaging in single cells, we demonstrated that only 6.9?±?0.8% of MSCs responded to noradrenaline by intracellular calcium release. Noradrenaline increases MSCs sensitivity to catecholamines in a transitory mode. Within 6?hrs after incubation with noradrenaline the proportion of cells responding by Ca(2+) release to the fresh noradrenaline addition has doubled but declined to the baseline after 24?hrs. Increased sensitivity was due to the elevated quantities of ?1A-adrenergic receptors on MSCs. Such elevation depended on the stimulation of ?-adrenergic receptors and adenylate cyclase activation. The data for the first time clarify mechanisms of regulation of MSCs sensitivity to noradrenaline.

SUBMITTER: Tyurin-Kuzmin PA 

PROVIDER: S-EPMC5011778 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Activation of β-adrenergic receptors is required for elevated α1A-adrenoreceptors expression and signaling in mesenchymal stromal cells.

Tyurin-Kuzmin Pyotr A PA   Fadeeva Julia I JI   Kanareikina Margarita A MA   Kalinina Natalia I NI   Sysoeva Veronika Yu VY   Dyikanov Daniyar T DT   Stambolsky Dmitriy V DV   Tkachuk Vsevolod A VA  

Scientific reports 20160906


Sympathetic neurons are important components of mesenchymal stem cells (MSCs) niche and noradrenaline regulates biological activities of these cells. Here we examined the mechanisms of regulation of MSCs responsiveness to noradrenaline. Using flow cytometry, we demonstrated that α1A adrenergic receptors isoform was the most abundant in adipose tissue-derived MSCs. Using calcium imaging in single cells, we demonstrated that only 6.9 ± 0.8% of MSCs responded to noradrenaline by intracellular calci  ...[more]

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