Dual-color plasmonic enzyme-linked immunosorbent assay based on enzyme-mediated etching of Au nanoparticles.

ABSTRACT: Colorimetric enzyme-linked immunosorbent assay utilizing 3'-3-5'-5-tetramethylbenzidine(TMB) as the chromogenic substrate has been widely used in the hospital for the detection of all kinds of disease biomarkers. Herein, we demonstrate a strategy to change this single-color display into dual-color responses to improve the accuracy of visual inspection. Our investigation firstly reveals that oxidation state of 3'-3-5'-5-tetramethylbenzidine (TMB(2+)) can quantitatively etch gold nanoparticles. Therefore, the incorporation of gold nanoparticles into a commercial TMB-based ELISA kit could generate dual-color responses: the solution color varied gradually from wine red (absorption peak located at ~530?nm) to colorless, and then from colorless to yellow (absorption peak located at ~450?nm) with the increase amount of targets. These dual-color responses effectively improved the sensitivity as well as the accuracy of visual inspection. For example, the proposed dual-color plasmonic ELISA is demonstrated for the detection of prostate-specific antigen (PSA) in human serum with a visual limit of detection (LOD) as low as 0.0093?ng/mL.

SUBMITTER: Guo L 

PROVIDER: S-EPMC5013390 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature