Unknown

Dataset Information

0

Co-infection with two strains of Brome mosaic bromovirus reveals common RNA recombination sites in different hosts.


ABSTRACT: We have previously reported intra-segmental crossovers in Brome mosaic virus (BMV) RNAs. In this work, we studied the homologous recombination of BMV RNA in three different hosts: barley (Hordeum vulgare), Chenopodium quinoa, and Nicotiana benthamiana that were co-infected with two strains of BMV: Russian (R) and Fescue (F). Our work aimed at (1) establishing the frequency of recombination, (2) mapping the recombination hot spots, and (3) addressing host effects. The F and R nucleotide sequences differ from each other at many translationally silent nucleotide substitutions. We exploited this natural variability to track the crossover sites. Sequencing of a large number of cDNA clones revealed multiple homologous crossovers in each BMV RNA segment, in both the whole plants and protoplasts. Some recombination hot spots mapped at similar locations in different hosts, suggesting a role for viral factors, but other sites depended on the host. Our results demonstrate the chimeric ('mosaic') nature of the BMV RNA genome.

SUBMITTER: Kolondam B 

PROVIDER: S-EPMC5014487 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

altmetric image

Publications

Co-infection with two strains of Brome mosaic bromovirus reveals common RNA recombination sites in different hosts.

Kolondam Beivy B   Rao Parth P   Sztuba-Solinska Joanna J   Weber Philipp H PH   Dzianott Aleksandra A   Johns Mitrick A MA   Bujarski Jozef J JJ  

Virus evolution 20151223 1


We have previously reported intra-segmental crossovers in Brome mosaic virus (BMV) RNAs. In this work, we studied the homologous recombination of BMV RNA in three different hosts: barley (<i>Hordeum vulgare)</i>, <i>Chenopodium quinoa</i>, and <i>Nicotiana benthamiana</i> that were co-infected with two strains of BMV: Russian (R) and Fescue (F). Our work aimed at (1) establishing the frequency of recombination, (2) mapping the recombination hot spots, and (3) addressing host effects. The F and R  ...[more]

Similar Datasets

| PRJNA718423 | ENA
| S-EPMC7114393 | biostudies-literature
| S-EPMC6430555 | biostudies-literature
| S-EPMC3468295 | biostudies-literature
| S-EPMC7187956 | biostudies-literature
| S-EPMC7059527 | biostudies-literature
| EMPIAR-10011 | biostudies-other
2010-12-01 | GSE19343 | GEO
2008-02-01 | GSE9693 | GEO
| S-EPMC8214199 | biostudies-literature