Ontology highlight
ABSTRACT:
SUBMITTER: Briand L
PROVIDER: S-EPMC5017159 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Briand L L Marcion G G Kriznik A A Heydel J M JM Artur Y Y Garrido C C Seigneuric R R Neiers F F
Scientific reports 20160909
Escherichia coli is an important experimental, medical and industrial cell factory for recombinant protein production. The inducible lac promoter is one of the most commonly used promoters for heterologous protein expression in E. coli. Isopropyl-β-D-thiogalactoside (IPTG) is currently the most efficient molecular inducer for regulating this promoter's transcriptional activity. However, limitations have been observed in large-scale and microplate production, including toxicity, cost and culture ...[more]