Unknown

Dataset Information

0

Exonuclease of human DNA polymerase gamma disengages its strand displacement function.


ABSTRACT: Pol ?, the only DNA polymerase found in human mitochondria, functions in both mtDNA repair and replication. During mtDNA base-excision repair, gaps are created after damaged base excision. Here we show that Pol ? efficiently gap-fills except when the gap is only a single nucleotide. Although wild-type Pol ? has very limited ability for strand displacement DNA synthesis, exo(-) (3'-5' exonuclease-deficient) Pol ? has significantly high activity and rapidly unwinds downstream DNA, synthesizing DNA at a rate comparable to that of the wild-type enzyme on a primer-template. The catalytic subunit Pol ?A alone, even when exo(-), is unable to synthesize by strand displacement, making this the only known reaction of Pol ? holoenzyme that has an absolute requirement for the accessory subunit Pol ?B.

SUBMITTER: He Q 

PROVIDER: S-EPMC5017585 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Exonuclease of human DNA polymerase gamma disengages its strand displacement function.

He Quan Q   Shumate Christie K CK   White Mark A MA   Molineux Ian J IJ   Yin Y Whitney YW  

Mitochondrion 20130830 6


Pol γ, the only DNA polymerase found in human mitochondria, functions in both mtDNA repair and replication. During mtDNA base-excision repair, gaps are created after damaged base excision. Here we show that Pol γ efficiently gap-fills except when the gap is only a single nucleotide. Although wild-type Pol γ has very limited ability for strand displacement DNA synthesis, exo(-) (3'-5' exonuclease-deficient) Pol γ has significantly high activity and rapidly unwinds downstream DNA, synthesizing DNA  ...[more]

Similar Datasets

| S-EPMC5041465 | biostudies-literature
| S-EPMC3783171 | biostudies-literature
| S-EPMC4216916 | biostudies-literature
| S-EPMC4417170 | biostudies-literature
| S-EPMC4557304 | biostudies-literature
| S-EPMC1088371 | biostudies-literature
| S-EPMC7119269 | biostudies-literature
| S-EPMC4856994 | biostudies-literature
| S-EPMC7863874 | biostudies-literature
| S-EPMC5672030 | biostudies-literature