Dataset Information

RBm?TX14 Increases the Life Span and Promotes the Locomotion of Caenorhabditis Elegans.

ABSTRACT: The scorpion has been extensively used in various pharmacological profiles or as food supplies. The exploration of scorpion venom has been reported due to the presence of recombinant peptides. rBm?TX14 is an ?-neurotoxin extracted from the venom gland of the East Asian scorpion Buthus martensii Karsch and can affect ion channel conductance. Here, we investigated the functions of rBm?TX14 using the Caenorhabditis elegans model. Using western blot analysis, rBm?TX14 was shown to be expressed both in the cytoplasm and inclusion bodies in the E.coli Rosetta (DE3) strain. Circular dichroism spectroscopy analysis demonstrated that purified rBm?TX14 retained its biological structures. Next, feeding nematodes with E.coli Rosetta (DE3) expressing rBm?TX14 caused extension of the life span and promoted the locomotion of the nematodes. In addition, we identified several genes that play various roles in the life span and locomotion of C. elegans through microarray analysis and quantitative real-time PCR. Furthermore, if the amino acid site H15 of rBm?TX14 was mutated, rBm?TX14 no longer promoted the C. elegans life span. In conclusion, the results not only demonstrated the functions and mechanism of rBm?TX14 in C. elegans, but also provided the new sight in the utility of recombinant peptides from scorpion venom.

SUBMITTER: Chen L

PROVIDER: S-EPMC5017660 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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rBmαTX14 Increases the Life Span and Promotes the Locomotion of Caenorhabditis Elegans.

Chen Lan L Zhang Ju J Xu Jie J Wan Lu L Teng Kaixuan K Xiang Jin J Zhang Rui R Huang Zebo Z Liu Yongmei Y Li Wenhua W Liu Xin X

PloS one 20160909 9

The scorpion has been extensively used in various pharmacological profiles or as food supplies. The exploration of scorpion venom has been reported due to the presence of recombinant peptides. rBmαTX14 is an α-neurotoxin extracted from the venom gland of the East Asian scorpion Buthus martensii Karsch and can affect ion channel conductance. Here, we investigated the functions of rBmαTX14 using the Caenorhabditis elegans model. Using western blot analysis, rBmαTX14 was shown to be expressed both ...[more]

PMID: 27611314

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Project description:Animal aging is characterized by progressive, degenerative changes in many organ systems. Because age-related degeneration is a major contributor to disability and death in humans, treatments that delay age-related degeneration are desirable. However, no drugs that delay normal human aging are currently available. To identify drugs that delay age-related degeneration, we used the powerful Caenorhabditis elegans model system to screen for FDA-approved drugs that can extend the adult lifespan of worms. Here we show that captopril extended mean lifespan. Captopril is an angiotensin-converting enzyme (ACE) inhibitor used to treat high blood pressure in humans. To explore the mechanism of captopril, we analyzed the acn-1 gene that encodes the C. elegans homolog of ACE. Reducing the activity of acn-1 extended the mean life span. Furthermore, reducing the activity of acn-1 delayed age-related degenerative changes and increased stress resistance, indicating that acn-1 influences aging. Captopril could not further extend the lifespan of animals with reduced acn-1, suggesting they function in the same pathway; we propose that captopril inhibits acn-1 to extend lifespan. To define the relationship with previously characterized longevity pathways, we analyzed mutant animals. The lifespan extension caused by reducing the activity of acn-1 was additive with caloric restriction and mitochondrial insufficiency, and did not require sir-2.1, hsf-1 or rict-1, suggesting that acn-1 functions by a distinct mechanism. The interactions with the insulin/IGF-1 pathway were complex, since the lifespan extensions caused by captopril and reducing acn-1 activity were additive with daf-2 and age-1 but required daf-16. Captopril treatment and reducing acn-1 activity caused similar effects in a wide range of genetic backgrounds, consistent with the model that they act by the same mechanism. These results identify a new drug and a new gene that can extend the lifespan of worms and suggest new therapeutic strategies for addressing age-related degenerative changes.

Dataset Information

RBm?TX14 Increases the Life Span and Promotes the Locomotion of Caenorhabditis Elegans.

Publications

rBmαTX14 Increases the Life Span and Promotes the Locomotion of Caenorhabditis Elegans.

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