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TreA Codifies for a Trehalase with Involvement in Xanthomonas citri subsp. citri Pathogenicity.


ABSTRACT: Citrus canker, caused by the bacterium Xanthomonas citri subsp. citri (Xcc), is a severe disease of citrus. Xcc presents broad spectrum of citrus hosts including economically important species whereas X. fuscans subsp. aurantifolii-type C (XauC) causes a milder disease and only infects Citrus aurantifolia. Trehalase catalyzes hydrolysis of the disaccharide trehalose, a sugar that has been reported to be related to Xcc pathogenicity. We expressed the recombinant gene product and assessed Xcc trehalase structural and kinetics data. The recombinant protein presented 42.7% of secondary structures in ?-helix and 13% in ?-sheets, no quaternary structure in solution, and Michaelis-Menten constant (KM) of 0.077 mM and Vmax 55.308 ?Mol glucose.min-1.mg protein-1 for trehalose. A Xcc mutant strain (Xcc?treA) was produced by gene deletion from Xcc genome. Enzymatic activity of trehalase was determined in Xcc, XauC and Xcc?treA cellular lysates, showing the highest values for XauC in in vitro infective condition and no activity for Xcc?treA. Finally, leaves of Citrus aurantifolia infected with Xcc?treA showed much more drenching and necrosis than those infected by wild type Xcc. We concluded that trehalase contributes to alleviate bacterial virulence and that inability for trehalose hydrolysis may promote higher Xcc infectivity.

SUBMITTER: Alexandrino AV 

PROVIDER: S-EPMC5017680 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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treA Codifies for a Trehalase with Involvement in Xanthomonas citri subsp. citri Pathogenicity.

Alexandrino André Vessoni AV   Goto Leandro Seiji LS   Novo-Mansur Maria Teresa Marques MT  

PloS one 20160909 9


Citrus canker, caused by the bacterium Xanthomonas citri subsp. citri (Xcc), is a severe disease of citrus. Xcc presents broad spectrum of citrus hosts including economically important species whereas X. fuscans subsp. aurantifolii-type C (XauC) causes a milder disease and only infects Citrus aurantifolia. Trehalase catalyzes hydrolysis of the disaccharide trehalose, a sugar that has been reported to be related to Xcc pathogenicity. We expressed the recombinant gene product and assessed Xcc treh  ...[more]

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