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Detection of Sphingomyelin Clusters by Raman Spectroscopy.

ABSTRACT: Sphingomyelin (SM) is a major sphingolipid in mammalian cells that forms specific lipid domains in combination with cholesterol (Chol). Using molecular-dynamics simulation and density functional theory calculation, we identified a characteristic Raman band of SM at ?1643 cm(-1) as amide I of the SM cluster. Experimental results indicate that this band is sensitive to the hydration of SM and the presence of Chol. We showed that this amide I Raman band can be utilized to examine the membrane distribution of SM. Similarly to SM, ceramide phosphoethanolamine (CerPE) exhibited an amide I Raman band in almost the same region, although CerPE lacks three methyl groups in the phosphocholine moiety of SM. In contrast to SM, the amide I band of CerPE was not affected by Chol, suggesting the importance of the methyl groups of SM in the SM-Chol interaction.

SUBMITTER: Shirota K

PROVIDER: S-EPMC5018142 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Detection of Sphingomyelin Clusters by Raman Spectroscopy.

Shirota Koichiro K Yagi Kiyoshi K Inaba Takehiko T Li Pai-Chi PC Murata Michio M Sugita Yuji Y Kobayashi Toshihide T

Biophysical journal 20160901 5

Sphingomyelin (SM) is a major sphingolipid in mammalian cells that forms specific lipid domains in combination with cholesterol (Chol). Using molecular-dynamics simulation and density functional theory calculation, we identified a characteristic Raman band of SM at ∼1643 cm(-1) as amide I of the SM cluster. Experimental results indicate that this band is sensitive to the hydration of SM and the presence of Chol. We showed that this amide I Raman band can be utilized to examine the membrane distr ...[more]

PMID: 27602727

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