Protective effects of parecoxib on rat primary astrocytes from oxidative stress induced by hydrogen peroxide.

ABSTRACT:

Objective

To investigate the protective effects of parecoxib from oxidative stress induced by hydrogen peroxide (H2O2) in rat astrocytes in vitro.

Methods

All experiments included 4 groups: (1) negative control (NC) group, without any treatment; (2) H2O2 treatment group, 100 μmol/L H2O2 treatment for 24 h; (3) and (4) parecoxib pretreatment groups, 80 and 160 μmol/L parecoxib treatment for 24 h, respectively, and then treated with 100 μmol/L H2O2. Several indices were investigated, and the expressions of Bax, Bcl-2, and brain-derived neurotrophic factor (BDNF) were quantified.

Results

Compared to the NC group, exposure to H2O2 resulted in significant morphological changes, which could be reversed by pretreatment of parecoxib. In addition, H2O2 treatment led to loss of

SUBMITTER: Ling YZ 

PROVIDER: S-EPMC5018616 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature