Analysis of single nucleotide polymorphisms based on RNA sequencing data of diverse bio-geographical accessions in barley.

ABSTRACT: Barley is one of the founder crops of Old world agriculture and has become the fourth most important cereal worldwide. Information on genome-scale DNA polymorphisms allows elucidating the evolutionary history behind domestication, as well as discovering and isolating useful genes for molecular breeding. Deep transcriptome sequencing enables the exploration of sequence variations in transcribed sequences; such analysis is particularly useful for species with large and complex genomes, such as barley. In this study, we performed RNA sequencing of 20 barley accessions, comprising representatives of several biogeographic regions and a wild ancestor. We identified 38,729 to 79,949 SNPs in the 19 domesticated accessions and 55,403 SNPs in the wild barley and revealed their genome-wide distributi

SUBMITTER: Takahagi K 

PROVIDER: S-EPMC5018957 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature