Ontology highlight
ABSTRACT:
SUBMITTER: Ishizuka T
PROVIDER: S-EPMC5020420 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Ishizuka Takumi T Liu Hong Shan HS Ito Kenichiro K Xu Yan Y
Scientific reports 20160913
Chromosome visualization is essential for chromosome analysis and genetic diagnostics. Here, we developed a click chemistry approach for multicolor imaging of chromosomal DNA instead of the traditional dye method. We first demonstrated that the commercially available reagents allow for the multicolor staining of chromosomes. We then prepared two pro-fluorophore moieties that served as light-up reporters to stain chromosomal DNA based on click reaction and visualized the clear chromosomes in mult ...[more]