Ontology highlight
ABSTRACT:
SUBMITTER: Ding L
PROVIDER: S-EPMC5023010 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Ding Li L Paszkowski-Rogacz Maciej M Winzi Maria M Chakraborty Debojyoti D Theis Mirko M Singh Sukhdeep S Ciotta Giovanni G Poser Ina I Roguev Assen A Chu Wai Kit WK Choudhary Chunaram C Mann Matthias M Stewart A Francis AF Krogan Nevan N Buchholz Frank F
Cell systems 20150826 2
We combine a genome-scale RNAi screen in mouse epiblast stem cells (EpiSCs) with genetic interaction, protein localization, and "protein-level dependency" studies-a systematic technique that uncovers post-transcriptional regulation-to delineate the network of factors that control the expression of Oct4, a key regulator of pluripotency. Our data signify that there are similarities, but also fundamental differences in Oct4 regulation in EpiSCs versus embryonic stem cells (ESCs). Through multiparam ...[more]