Ontology highlight
ABSTRACT:
SUBMITTER: Veshaguri S
PROVIDER: S-EPMC5023152 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Veshaguri Salome S Christensen Sune M SM Kemmer Gerdi C GC Ghale Garima G Møller Mads P MP Lohr Christina C Christensen Andreas L AL Justesen Bo H BH Jørgensen Ida L IL Schiller Jürgen J Hatzakis Nikos S NS Grabe Michael M Pomorski Thomas Günther TG Stamou Dimitrios D
Science (New York, N.Y.) 20160301 6280
In eukaryotes, P-type adenosine triphosphatases (ATPases) generate the plasma membrane potential and drive secondary transport systems; however, despite their importance, their regulation remains poorly understood. We monitored at the single-molecule level the activity of the prototypic proton-pumping P-type ATPase Arabidopsis thaliana isoform 2 (AHA2). Our measurements, combined with a physical nonequilibrium model of vesicle acidification, revealed that pumping is stochastically interrupted by ...[more]