Synthesis, characterization, and application of polysodium N-alkylenyl ?-d-glucopyranoside surfactants for micellar electrokinetic chromatography-tandem mass spectrometry.

ABSTRACT: Sugar-based ionic surfactants forming micelles are known to suppress ESI of various compounds due to decrease in surface tension upon micelle formation . For the first time, poly (sodium N-undecylenyl-?-d-glucopyranoside 4,6-hydrogen phosphate, (poly-?-d-SUGP) based surfactants with different chain lengths and head groups have been successfully synthesized, characterized, and applied as compatible chiral selector for MEKC-ESI-MS/MS. First, the effect of polymerization concentration of the monomer, ?-d-SUGP, was evaluated by enantioseparation of one anionic compound (1,1'-binaphthyl-2,2'diyl-hydrogen phosphate) and one zwitterionic compound (dansylated phenylalanine) in MEKC-UV to find the optimum molar surfactant concentration for polymerization. Next, MEKC-UV and MEKC-MS were compared for the enantioseparation of 1,1'-binaphthyl-2,2'diyl-hydrogen phosphate. The influence of polymeric glucopyranoside based surfactant head groups and carbon chain lengths on chiral Rs was evaluated for two classes of cationic drugs (ephedrine alkaloids and ?-blockers). Finally, enantioselective MEKC-MS of ephedrine alkaloids and ?-blockers were profiled at their optimum pH 5.0 and 7.0, respectively, using 20 mM NH4 OAc, 25 mM poly-?-d-SUGP at 30 kV and 25°C under optimum spray chamber conditions. The LOD for most of the enantiomers ranges from 10 to 100 ng/mL with S/N of at least ?3.0.

SUBMITTER: Liu Y 

PROVIDER: S-EPMC5023327 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature