Dataset Information

The utility of urine-circulating miRNAs for detection of prostate cancer.

ABSTRACT:

Background

In this paper, the utility of urine-circulating microRNAs (miRNAs) as the potential biomarker of prostate cancer (PCa), the second most prevalent male cancer worldwide, was evaluated.

Methods

Cancerous (N=56) and non-cancerous (N=16) prostate tissues were analysed on TaqMan Low Density Array, with the initial screening of 754 miRNAs in a subset of the samples. The abundance of selected miRNAs was analysed in urine specimens from two independent cohorts of patients with PCa (N=215 overall), benign prostatic hyperplasia (BPH; N=23), and asymptomatic controls (ASC; N=62) by means of quantitative reverse transcription PCR.

Results

Over 100 miRNAs were found deregulated in PCa as compared with non-cancerous prostate tissue. After thorough validation, four miRNAs were selected for the analysis in urine specimens. The abundance of miR-148a and miR-375 in urine was identified as specific biomarkers of PCa in both cohorts. Combined analysis of urine-circulating miR-148a and miR-375 was highly sensitive and specific for PCa in both cohorts (AUC=0.79 and 0.84) and strongly improved the diagnostic power of the PSA test (AUC=0.85, cohort PCa1), including the grey diagnostic zone (AUC=0.90).

Conclusions

Quantitative measurement of urine-circulating miR-148a and miR-375 can serve as the non-invasive tool for sensitive and specific detection of PCa.

SUBMITTER: Stuopelyte K

PROVIDER: S-EPMC5023772 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Publications

The utility of urine-circulating miRNAs for detection of prostate cancer.

Stuopelyte Kristina K Daniunaite Kristina K Bakavicius Arnas A Lazutka Juozas R JR Jankevicius Feliksas F Jarmalaite Sonata S

British journal of cancer 20160804 6

<h4>Background</h4>In this paper, the utility of urine-circulating microRNAs (miRNAs) as the potential biomarker of prostate cancer (PCa), the second most prevalent male cancer worldwide, was evaluated.<h4>Methods</h4>Cancerous (N=56) and non-cancerous (N=16) prostate tissues were analysed on TaqMan Low Density Array, with the initial screening of 754 miRNAs in a subset of the samples. The abundance of selected miRNAs was analysed in urine specimens from two independent cohorts of patients with ...[more]

PMID: 27490805

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Dataset Information

The utility of urine-circulating miRNAs for detection of prostate cancer.

Background

Methods

Results

Conclusions

Publications

The utility of urine-circulating miRNAs for detection of prostate cancer.

Similar Datasets

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