Transition from physical activity to inactivity increases skeletal muscle miR-148b content and triggers insulin resistance.
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ABSTRACT:
SUBMITTER: Gastebois C
PROVIDER: S-EPMC5027343 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
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Transition from physical activity to inactivity increases skeletal muscle miR-148b content and triggers insulin resistance.
Gastebois Caroline C Chanon Stéphanie S Rome Sophie S Durand Christine C Pelascini Elise E Jalabert Audrey A Euthine Vanessa V Pialoux Vincent V Blanc Stéphane S Simon Chantal C Lefai Etienne E
Physiological reports 20160901 17
This study investigated miR-148b as a potential physiological actor of physical inactivity-induced effects in skeletal muscle. By using animal and human protocols, we demonstrated that the early phase of transition toward inactivity was associated with an increase in muscle miR-148b content, which triggered the downregulation of NRAS and ROCK1 target genes. Using human myotubes, we demonstrated that overexpression of miR-148b decreased NRAS and ROCK1 protein levels, and PKB phosphorylation and g ...[more]
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