Ontology highlight
ABSTRACT:
SUBMITTER: Gould GM
PROVIDER: S-EPMC5029451 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
RNA (New York, N.Y.) 20160729 10
Spliced messages constitute one-fourth of expressed mRNAs in the yeast Saccharomyces cerevisiae, and most mRNAs in metazoans. Splicing requires 5' splice site (5'SS), branch point (BP), and 3' splice site (3'SS) elements, but the role of the BP in splicing control is poorly understood because BP identification remains difficult. We developed a high-throughput method, Branch-seq, to map BPs and 5'SSs of isolated RNA lariats. Applied to S. cerevisiae, Branch-seq detected 76% of expressed, annotate ...[more]