Ontology highlight
ABSTRACT:
SUBMITTER: Zheng D
PROVIDER: S-EPMC5029459 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Zheng Dinghai D Liu Xiaochuan X Tian Bin B
RNA (New York, N.Y.) 20160810 10
Sequencing of the 3' end of poly(A)(+) RNA identifies cleavage and polyadenylation sites (pAs) and measures transcript expression. We previously developed a method, 3' region extraction and deep sequencing (3'READS), to address mispriming issues that often plague 3' end sequencing. Here we report a new version, named 3'READS+, which has vastly improved accuracy and sensitivity. Using a special locked nucleic acid oligo to capture poly(A)(+) RNA and to remove the bulk of the poly(A) tail, 3'READS ...[more]