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The PRKD1 promoter is a target of the KRas-NF-?B pathway in pancreatic cancer.

ABSTRACT: Increased expression of PRKD1 and its gene product protein kinase D1 (PKD1) are linked to oncogenic signaling in pancreatic ductal adenocarcinoma, but a direct functional relationship to oncogenic KRas has not been established so far. We here describe the PRKD1 gene promoter as a target for oncogenic KRas signaling. We demonstrate that KRas-induced activation of the canonical NF-?B pathway is one mechanism of how PRKD1 expression is increased and identify the binding sites for NF-?B in the PRKD1 promoter. Altogether, these results describe a novel mechanism governing PRKD1 gene expression in PDA and provide a functional link between oncogenic KRas, NF-?B and expression of PRKD1.

SUBMITTER: Doppler H

PROVIDER: S-EPMC5030668 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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The PRKD1 promoter is a target of the KRas-NF-κB pathway in pancreatic cancer.

Döppler Heike H Panayiotou Richard R Reid Elizabeth M EM Maimo Willibroad W Bastea Ligia L Storz Peter P

Scientific reports 20160921

Increased expression of PRKD1 and its gene product protein kinase D1 (PKD1) are linked to oncogenic signaling in pancreatic ductal adenocarcinoma, but a direct functional relationship to oncogenic KRas has not been established so far. We here describe the PRKD1 gene promoter as a target for oncogenic KRas signaling. We demonstrate that KRas-induced activation of the canonical NF-κB pathway is one mechanism of how PRKD1 expression is increased and identify the binding sites for NF-κB in the PRKD1 ...[more]

PMID: 27649783

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