Ontology highlight
ABSTRACT:
SUBMITTER: Chen WW
PROVIDER: S-EPMC5030821 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Chen Walter W WW Freinkman Elizaveta E Wang Tim T Birsoy Kıvanç K Sabatini David M DM
Cell 20160801 5
Mitochondria house metabolic pathways that impact most aspects of cellular physiology. While metabolite profiling by mass spectrometry is widely applied at the whole-cell level, it is not routinely possible to measure the concentrations of small molecules in mammalian organelles. We describe a method for the rapid and specific isolation of mitochondria and use it in tandem with a database of predicted mitochondrial metabolites ("MITObolome") to measure the matrix concentrations of more than 100 ...[more]