Ontology highlight
ABSTRACT:
SUBMITTER: Duchi D
PROVIDER: S-EPMC5031556 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Duchi Diego D Bauer David L V DL Fernandez Laurent L Evans Geraint G Robb Nicole N Hwang Ling Chin LC Gryte Kristofer K Tomescu Alexandra A Zawadzki Pawel P Morichaud Zakia Z Brodolin Konstantin K Kapanidis Achillefs N AN
Molecular cell 20160908 6
In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter. The mechanism of initial transcription is unclear due to the presence of transient intermediates and molecular heterogeneity. Here, we studied initial transcription on a lac promoter using single-molecule fluorescence observations of DNA scrunching on immobilized transcription complexes. Our work revealed a long pause ("initi ...[more]