Dataset Information
Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis.
Ontology highlight
ABSTRACT: Objective
DNA methylation may be a stable epigenetic contributor to defining fat cell lineage.Methods
We performed reduced representation bisulfite sequencing (RRBS) and RNA-seq to depict a genome-wide integrative view of the DNA methylome and transcriptome during brown and white adipogenesis.Results
Our analysis demonstrated that DNA methylation is a stable epigenetic signature for brown and white cell lineage before, during, and after differentiation. We identified 31 genes whose promoters were significantly differentially methylated between white and brown adipogenesis at all three time points of differentiation. Among them, five genes belong to the Hox family; their expression levels were anti-correlated with promoter methylation, suggesting a regulatory role of DNA methylation in transcription. Blocking DNA methylation with 5-Aza-cytidine increased the expression of these genes, with the most prominent effect on Hoxc10, a repressor of BAT marker expression.Conclusions
Our data suggest that DNA methylation may play an important role in lineage-specific development in adipocytes.
SUBMITTER: Lim YC
PROVIDER: S-EPMC5034609 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Publications
Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis.
Molecular metabolism 20160817 10
<h4>Objective</h4>DNA methylation may be a stable epigenetic contributor to defining fat cell lineage.<h4>Methods</h4>We performed reduced representation bisulfite sequencing (RRBS) and RNA-seq to depict a genome-wide integrative view of the DNA methylome and transcriptome during brown and white adipogenesis.<h4>Results</h4>Our analysis demonstrated that DNA methylation is a stable epigenetic signature for brown and white cell lineage before, during, and after differentiation. We identified 31 g ...[more]
