Adaptation of Mycobacterium tuberculosis to Impaired Host Immunity in HIV-Infected Patients.

ABSTRACT:

Background

It is unknown whether immunosuppression influences the physiologic state of Mycobacterium tuberculosis in vivo. We evaluated the impact of host immunity by comparing M. tuberculosis and human gene transcription in sputum between human immunodeficiency virus (HIV)-infected and uninfected patients with tuberculosis.

Methods

We collected sputum specimens before treatment from Gambians and Ugandans with pulmonary tuberculosis, revealed by positive results of acid-fast bacillus smears. We quantified expression of 2179 M. tuberculosis genes and 234 human immune genes via quantitative reverse transcription-polymerase chain reaction. We summarized genes from key functional categories with significantly increased or decreased expression.

Results

A total of 24 of 65

SUBMITTER: Walter ND 

PROVIDER: S-EPMC5034964 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature