Protein-protein interaction analysis for functional characterization of helicases.
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ABSTRACT:
SUBMITTER: Zybailov BL
PROVIDER: S-EPMC5035562 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Publications
Protein-protein interaction analysis for functional characterization of helicases.
Zybailov Boris L BL Byrd Alicia K AK Glazko Galina V GV Rahmatallah Yasir Y Raney Kevin D KD
Methods (San Diego, Calif.) 20160420
Helicases are enzymes involved in nucleic acid metabolism, playing major roles in replication, transcription, and repair. Defining helicases oligomerization state and transient and persistent protein interactions is essential for understanding of their function. In this article we review current methods for the protein-protein interaction analysis, and discuss examples of its application to the study of helicases: Pif1 and DDX3. Proteomics methods are our main focus - affinity pull-downs and che ...[more]
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