Ontology highlight
ABSTRACT:
SUBMITTER: Pharo HD
PROVIDER: S-EPMC5037394 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Pharo Heidi D HD Honne Hilde H Vedeld Hege M HM Dahl Christina C Andresen Kim K Liestøl Knut K Jeanmougin Marine M Guldberg Per P Lind Guro E GE
Scientific reports 20160927
Diverging methylation frequencies are often reported for the same locus in the same disease, underscoring the need for limiting technical variability in DNA methylation analyses. We have investigated seven likely sources of variability at different steps of bisulfite PCR-based DNA methylation analyses using a fully automated quantitative methylation-specific PCR setup of six gene promoters across 20 colon cancer cell lines. Based on >15,000 individual PCRs, all tested parameters affected the nor ...[more]