De Novo Characterization of Genes That Contribute to High-Level Ciprofloxacin Resistance in Escherichia coli.
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ABSTRACT:
SUBMITTER: Tran T
PROVIDER: S-EPMC5038283 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
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De Novo Characterization of Genes That Contribute to High-Level Ciprofloxacin Resistance in Escherichia coli.
Tran Thu T Ran Qinghong Q Ostrer Lev L Khodursky Arkady A
Antimicrobial agents and chemotherapy 20160923 10
Sensitization of resistant bacteria to existing antibiotics depends on the identification of candidate targets whose activities contribute to resistance. Using a transposon insertion library in an Escherichia coli mutant that was 2,000 times less susceptible to ciprofloxacin than its parent and the relative fitness scores, we identified 19 genes that contributed to the acquired ciprofloxacin resistance and mapped the shortest genetic path that increased the antibiotic susceptibility of the resis ...[more]
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