Ontology highlight
ABSTRACT:
SUBMITTER: Kandiba L
PROVIDER: S-EPMC5041007 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Kandiba Lina L Eichler Jerry J
Life (Basel, Switzerland) 20160803 3
Haloferax volcanii AglM and Halobacterium salinarum VNG1048G are UDP-glucose dehydrogenases involved in N-glycosylation in each species. Despite sharing >60% sequence identity and the ability of VNG1048G to functionally replace AglM in vivo, these proteins behaved differently as salinity changed. Whereas AglM was active in 2-4 M NaCl, VNG1048G lost much of its activity when salinity dropped below 3 M NaCl. To understand the molecular basis of this phenomenon, each protein was examined by size ex ...[more]