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Casein kinase 1 is recruited to nuclear speckles by FAM83H and SON.


ABSTRACT: In some fibroblasts, casein kinase 1? (CK1?) is localized to nuclear speckles, which are sub-nuclear compartments supplying splicing factors, whereas it is recruited on keratin filaments in colorectal cancer cells such as DLD1 cells. In order to obtain a deeper understanding of why CK1? is localized to these different subcellular sites, we herein elucidated the mechanisms underlying its localization to nuclear speckles. CK1? and FAM83H were localized to nuclear speckles in RKO and WiDr colorectal cancer cells, which do not express simple epithelial keratins, and in DLD1 cells transfected with siRNAs for type I keratins. The localization of FAM83H to nuclear speckles was also detected in colorectal cancer cells with a poorly organized keratin cytoskeleton in colorectal cancer tissues. Using an interactome analysis of FAM83H, we identified SON, a protein present in nuclear speckles, as a scaffold protein to which FAM83H recruits CK1?. This result was supported by the knockdown of FAM83H or SON delocalizing CK1? from nuclear speckles. We also found that CK1? and ? are localized to nuclear speckles in a FAM83H-dependent manner. These results suggest that CK1 is recruited to nuclear speckles by FAM83H and SON in the absence of an intact keratin cytoskeleton.

SUBMITTER: Kuga T 

PROVIDER: S-EPMC5041083 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Casein kinase 1 is recruited to nuclear speckles by FAM83H and SON.

Kuga Takahisa T   Kume Hideaki H   Adachi Jun J   Kawasaki Naoko N   Shimizu Maiko M   Hoshino Isamu I   Matsubara Hisahiro H   Saito Youhei Y   Nakayama Yuji Y   Tomonaga Takeshi T  

Scientific reports 20160929


In some fibroblasts, casein kinase 1α (CK1α) is localized to nuclear speckles, which are sub-nuclear compartments supplying splicing factors, whereas it is recruited on keratin filaments in colorectal cancer cells such as DLD1 cells. In order to obtain a deeper understanding of why CK1α is localized to these different subcellular sites, we herein elucidated the mechanisms underlying its localization to nuclear speckles. CK1α and FAM83H were localized to nuclear speckles in RKO and WiDr colorecta  ...[more]

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